Mouse RAPGEF2(Rap Guanine Nucleotide Exchange Factor 2) EIA Kit

Target Synonym:

NA

Target Species:

Mouse

UNIProt ID:

Q8CHG7

Target Name:

RAPGEF2

Type:

Sandwich

Sensitivity:

9.38 pg/mL

Detection Type:

Colormetric

Latin name:

Mus musculus

Detection Range:

15.625~1000pg/mL

Tested Sample Types:

Serum, Plasma, Cell supernatant

Properties:

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay

Product Name:

Mouse RAPGEF2(Rap Guanine Nucleotide Exchange Factor 2) EIA Kit

Test:

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Description:

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

Test principle:

This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse RAPGEF2. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RAPGEF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RAPGEF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse RAPGEF2. You can calculate the concentration of Mouse RAPGEF2 in the samples by comparing the OD of the samples to the standard curve.