Rat ChT(High Affinity Choline Transporter) ELISA Kit

Assay length:

3h

Sensitivity:

28pg/mL

Assay Type:

Sandwich

Standard:

5000pg/mL

Detection range:

78.12-5000pg/mL

Latin name:

Rattus norvegicus

Research Area:

Signal transduction;

Properties:

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names:

SLC5A7; hCHT, CHT1; Solute Carrier Family 5 Member 7,Choline Transporter; Hemicholinium-3-sensitive choline transporter

Test:

A high affinity purification column was use to purify ChT( Choline Transporter) ELISA Kit by ELK Biotech by chromatographic size exclusion.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About:

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Affinity Choline Transporter (ChT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Affinity Choline Transporter (ChT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Affinity Choline Transporter (ChT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Affinity Choline Transporter (ChT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.