Goat anti-Donkey IgG (H&L) - Affinity Pure, FITC Conjugate[IgG (H&L)]

Conjugation:

Anti-FITC Antibody

Products_type:

Secondary Antibody

Additional isotype:

Heavy & Light chains,IgG

Latin name:

Equus africanus asinus,Capra aegagrus hircus

Reactivity:

Donkey.Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin donkey serum immunoglobulins

Test:

A high affinity purification column was use to purify Goat anti- IgG (H&L) - Pure, FITC Conjugate[IgG (H&L)] by MyBioSource by chromatographic size exclusion.

Description:

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The ultrapure Goat anti- IgG (H&L) - Pure, FITC Conjugate[IgG (H&L)] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Properties:

This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.