Goat anti-Human IgG Fc, F(ab)'2 Fragment - Affinity Pure, Biotin Conjugate[IgG Fc, F(ab)'2 Fragment]

Conjugation:

Biotinylated

Products_type:

Secondary Antibody

Latin name:

Capra aegagrus hircus

Reactivity:

Human.Cross Reactivity: Based on IEP, no reactivity is observed to:non-immunoglobulin human serum immunoglobulinslight chains on all human immunoglobulins

Test:

A high affinity purification column was use to purify Goat anti- IgG Fc, F(ab)'2 - Pure, Conjugate[IgG Fc, F(ab)'2 ] by MyBioSource by chromatographic size exclusion.MyBioSource supplies antibodies that are for research of human proteins.

Additional isotype:

Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.IgG

Description:

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The ultrapure Goat anti- IgG Fc, F(ab)'2 - Pure, Conjugate[IgG Fc, F(ab)'2 ] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Properties:

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.