Donkey anti-Rat IgG (H&L) - Affinity Pure, Biotin Conjugate[IgG (H&L)]

Conjugation:

Biotinylated

Products_type:

Secondary Antibody

Additional isotype:

Heavy & Light chains,IgG

Latin name:

Equus africanus asinus,Rattus norvegicus

Reactivity:

Rat.Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin rat serum proteins

Test:

A high affinity purification column was use to purify anti- IgG (H&L) - Pure, Conjugate[IgG (H&L)] by MyBioSource by chromatographic size exclusion.

Properties:

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Description:

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The ultrapure anti- IgG (H&L) - Pure, Conjugate[IgG (H&L)] supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.