chicken polyclonal anti-Green Fluorescent Protein (GFP)[Neisseria gonorrhoeae] (not affinity purified) protein antibody

French translation:

anticorps

Disease:

neisseria

Group:

Polyclonals and antibodies

Antigen:

Green Fluorescent Protein (GFP)[Neisseria gonorrhoeae] (not affinity purified)

Test:

A high affinity purification column was use to purify polyclonal anti- Protein (GFP)[Neisseria gonorrhoeae] (not ) protein antibody by gallus IgY by chromatographic size exclusion.

About:

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Properties:

If you buy Antibodies supplied by gallus IgY they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.

Chicken IgY:

Gallus gallus domesticus and other IgY antibodies have high affinity and are easy to collect from the yolk. Gallus gallus can produce 20 more than in rabbit polyclonal. The antibody will not bind to rheumatoid factor or protein or Fc regions. This gives very much reduced background very much suited for diagnostic applications. Papain will digest IgY’s to divalent Fab antibody fragments. IgY’s are suited for Elisa kit development, Immunohistochemistry and Western Blot.

Description:

1 of protein A or G purified by gallus IgY will give more specificity than crude serum.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.