MARK1/2/3/4 (G152) polyclonal antibody

Applications:

WB

BiowMW:

~ 85 kDa

French translation:

anticorps

Reactivity:

Human,Mouse,Rat

Application_all:

WB: 1:1000~1:2000

Group:

Polyclonals and antibodies

Swiss-Prot:

Q9P0L2/Q7KZI7/P27448/Q96L34

Immunogen:

Synthetic peptide, corresponding to Human GPR116.

Note:

For research use only, not for use in diagnostic procedure.

Specificity:

MARK1/2/3/4 pAb detects endogenous levels of MARK1/2/3/4 protein.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name:

Adhesion G protein-coupled receptor F5; G-protein coupled receptor 116; ADGRF5; GPR116; KIAA0758

Properties:

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Usage:

Western blot (WB) analysis of MARK1/2/3/4 (G152) pAb at 1:1000 dilution Lane1:The Brain tissue lysate of Rat(40ug) Lane2:The Heart tissue lysate of Mouse(5ug) Lane3:L02 whole cell lysate(30ug) Lane4:HEK293T whole cell lysate(30ug)

About:

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.