VAV2 (D138) polyclonal antibody

Swiss-Prot:

P52735

BiowMW:

~108 kDa

French translation:

anticorps

Reactivity:

Human,Rat

Applications:

WB IHC IF

Group:

Polyclonals and antibodies

Application_all:

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Alternative Name:

Guanine nucleotide exchange factor VAV2; VAV-2; VAV2

Note:

For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to amino acids 100-150 of Human VAV2.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Specificity:

VAV2 (D138) polyclonal antibody detects endogenous levels of VAV2 protein.

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Properties:

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Usage:

Western blot (WB) analysis of VAV2 (D138) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(20ug) Lane2:Hela whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug)

About:

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.