Vav1 (phospho-Y174) polyclonal antibody

Swiss-Prot:

P15498

Applications:

WB IHC

French translation:

anticorps

Reactivity:

Human,Rat

BiowMW:

~ 100 kDa

Group:

Polyclonals and antibodies

Alternative Name:

Proto-oncogene vav; VAV1; VAV

Application_all:

WB: 1:500~1:1000 IHC: 1:50~1:200

Note:

For research use only, not for use in diagnostic procedure.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Synthetic phosphopeptide derived from human VaV1 around the phosphorylation site of Tyrosine 174.

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C long term._x000D_ Avoid freeze-thaw cycles.

Specificity:

p-Vav1 (Y174) polyclonal antibody detects endogenous levels of Vav1 protein when phosphorylated at Tyr174.

Properties:

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity:

The antibody was affinity-purified from rabbit antiserum by_x000D_ affinity-chromatography using epitope-specific immunogen_x000D_ and the purity is > 95% (by SDS-PAGE).

Usage:

Western blot (WB) analysis of p-Vav1 (Y174) pAb at 1:500 dilution Lane1:THP-1 whole cell lysate(40ug) Lane2:HuT78 whole cell lysate(40ug) Lane3:The Spleen tissue lysate of Mouse(40ug) Lane4:The Spleen tissue lysate of Rat(40ug)

About:

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.