RAPGEF3 polyclonal antibody

Swiss-Prot:

O95398

Applications:

WB ICC

BiowMW:

~ 99 kDa

French translation:

anticorps

Reactivity:

Human,Mouse,Rat

Group:

Polyclonals and antibodies

Application_all:

WB: 1:500~1:1000 ICC: 1:50~1:200

Note:

For research use only, not for use in diagnostic procedure.

Product:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Specificity:

RAPGEF3 polyclonal antibody detects endogenous levels of RAPGEF3 protein.

Immunogen:

Synthetic peptide, corresponding to amino acids 799-844 of Human RAPGEF3.

Storage&Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Properties:

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Usage:

Western blot (WB) analysis of RAPGEF3 polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate

Purification&Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

About:

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Alternative Name:

Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; cAMP-regulated guanine nucleotide exchange factor I; cAMP-GEFI; CGEF1; EPAC; EPAC1