Mouse Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III ELISA Kit

Additional isotype:

IgG

Assay procedure:

1-4h

Species reactivity:

Mouse

Detection wavelengt:

450 nm

Tissue:

region

Latin name:

Mus musculus

Applications:

Enzyme Linked Immunosorbent Assay

Antigen:

Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III

Synonym name:

Murine Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III ELISA Kit

Long name:

ELISA test kit for Mouse Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III

Properties:

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

General description:

This is a ready to use kit for performing ELISA tests for detection of Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III in samples from Mouse

Sample types:

Our Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III ELISA kit has been proven to work and successfully detect Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III in Murine

Shipping, storage, shelf life:

The Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Mouse Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III ELISA Kit is 6 months after delivery.

Kit contents:

ELISA test kit for Mouse Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor IIIAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Description:

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.

Test:

A high affinity purification column was use to purify Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III ELISA by SAB ELISA KIT by chromatographic size exclusion.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Tips:

Due to transportation and handling small amounts of the liquid components of the Murine Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.

Summary of the prodecure:

1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.