1x96 well plate
EK0912
557 EUR
Entrez nr.:
2208
Minutes of TMB incubation:
25-30
Gen protein identity:
P06734
ELISA's sensitivity:
< 10pg/ml
Gene tested:
Human CD23
Antigen:
NSO, D48-S321
Anti-coagulation:
heparin or EDTA
ELISA kit detection limits:
31.2pg/ml to 2000pg/ml
Target of this ELISA:
Natural and recombinant human CD23
Uniprot:
Low affinity immunoglobulin epsilon Fc receptor
Gene name:
Fc fragment of IgE, low affinity II, receptor for (CD23)
Test:
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Detector and capure antibodies:
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
Cross reaction:
the ELISA does not cross react with any other proteins except from closely related species
Keep at:
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
ELISA's application:
For quantitative measurement of human CD23 in cell culture supernatant, serum, plasma heparin, EDTA and urine.
ELISA standard curve:
Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.012 + 0.052 + 0.109 + 0.200 + 0.403 + 0.660 + 1.477 + 2.083
ELISA's precision parameters:
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);132;288;589;139;293;602 ELISA standard deviations 11.35;18.72;43;12.79;24.61;45.75 CV(%);8.6;6.5;7.3;9.2;8.4;7.6
Alternative:
BLAST 2;BLAST-2;C-type lectin domain family 4 member J;CD23;CD23 antigen;CD23A;CLEC4J;Fc fragment of IgE low affinity II receptor for (CD23);Fc-epsilon-RII;FCE2;Fcer2;FCER2_HUMAN;IGEBF;Immunoglobulin E-binding factor;Low affinity immunoglobulin epsilon Fc receptor;Low affinity immunoglobulin epsilon Fc receptor soluble form;Lymphocyte IgE receptor
How the elisa works:
Sample the amount of 100ul/well of the standard solution at 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human CD23 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum, plasma( heparin, EDTA) or urine to the ELISA plate. Keep in mind that each human CD23 standard solution and each sample be measured in duplicate.
The ELISA contains:
Catalog number;Description;Quantity -;96-well plate precoated with anti- human CD23 antibodies;1 ST0000-10;Lyophilized recombinant human CD23 standard;10ng/tube×2 AR1107;Biotinylated anti- human CD23 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Properties:
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA kit description:
GENTAUR's human CD23 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for CD23 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, D48-S321) and your samples are measured with a biotin conjugated polyclonal from goat specific for CD23 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human CD23 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
More gene info:
CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibodies isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23(FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.