Size

1x96 well plate

Catalog no.

EK1312

Price

596 EUR

Minutes of TMB incubation:

25-30

Entrez nr.:

2213&9103

ELISA's sensitivity:

< 10pg/ml

Gene tested:

human CD32

Gen protein identity:

P31994&P31995

Antigen:

NSO, A46-P217

Anti-coagulation:

heparin or EDTA

ELISA kit detection limits:

46.9pg/ml to 3000pg/ml

Target of this ELISA:

Natural and recombinant human CD32

Gene name:

Fc fragment of IgG, low affinity IIb, receptor (CD32)

Uniprot:

Low affinity immunoglobulin gamma Fc region receptor II-b

Test:

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Detector and capure antibodies:

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

Cross reaction:

the ELISA does not cross react with any other proteins except from closely related species

Keep at:

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

ELISA's application:

For quantitative measurement of human CD32 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .

ELISA standard curve:

Concentration(pg/ml) + 0 + 46.9 + 93.8 + 187.5 + 375 + 750 + 1500 + 3000 O.D. + 0.007 + 0.124 + 0.199 + 0.255 + 0.541 + 0.813 + 1.408 + 2.266

ELISA's precision parameters:

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);147;890;1865;168;904;2162 ELISA standard deviations 6.62;32.93;80.2;9.75;41.6;112.42 CV(%);4.5;3.7;4.3;5.8;4.6;5.2

More gene info:

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibodies production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

The ELISA contains:

Catalog number;Description;Quantity -;96-well plate precoated with anti- human CD32 antibodies;1 ST0000-10;Lyophilized recombinant human CD32 standard;10ng/tube×2 AR1107;Biotinylated anti- human CD32 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

Properties:

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA kit description:

GENTAUR's human CD32 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for CD32 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, A46-P217) and your samples are measured with a biotin conjugated polyclonal from goat specific for CD32 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human CD32 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

How the elisa works:

Sample the amount of 100ul/well of the standard solution at 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.8pg/ml, 46.9pg/ml human CD32 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each human CD32 standard solution and each sample is measured in duplicate.

Alternative:

CD 32;CD32;CD32 antigen;CD32A;CD32B;CD32C;CDw32;Fc fragment of IgG low affinity II receptor for (CD32);Fc fragment of IgG low affinity IIa receptor;Fc fragment of IgG low affinity IIa receptor for (CD32);Fc fragment of IgG low affinity IIb receptor;Fc fragment of IgG low affinity IIb receptor for (CD32);Fc fragment of IgG low affinity IIc receptor;Fc fragment of IgG low affinity IIc receptor for (CD32);Fc gamma receptor IIB;Fc gamma RII a;Fc gamma RII b;Fc gamma RII c;Fc gamma RIIa;Fc gamma RIIb ;Fc gamma RIIc;Fc receptor, IgG, low affinity IIb;Fc-gamma RII-a;Fc-gamma RII-b;Fc-gamma RII-c;Fc-gamma-RIIa;FC-gamma-RIIB;Fc-GAMMA-RIIC;Fc[g]RII;FCG 2;FCG2;FCG2A_HUMAN;FcgammaRIIC;FCGR 2;FcGR;FCGR2;FCGR2A;FCGR2A1;FCGR2B;FCGR2C;Fcgr3;Fcgr3a;FcgRII;Fcr-2;Fcr-3;FcRII a;FCRII;FcRII b;FcRII c;FcRII-a;FcRIIC;IGFR 2;IGFR2;IgG Fc receptor II a;IgG Fc receptor II b;IgG Fc receptor II beta;IgG Fc receptor II c;IgG Fc receptor II-a;IgG Fc receptor II-b;IgG Fc receptor II-c;Immunoglobulin G Fc receptor II;Low affinity;immunoglobulin gamma Fc region receptor II a;Low affinity immunoglobulin gamma Fc region receptor II;Low affinity immunoglobulin gamma Fc region receptor II b;Low affinity immunoglobulin gamma Fc region receptor II c;Low affinity immunoglobulin gamma Fc region receptor II-a;Low affinity immunoglobulin gamma Fc region receptor II-b;Low affinity immunoglobulin gamma Fc region receptor II-c;Ly-17;Ly-m20;LyM-1;Lymphocyte antigen 17;MGC23887;MGC30032