1x96 well plate
EK1313
596 EUR
Entrez nr.:
14130
Minutes of TMB incubation:
20-25
Gen protein identity:
P08101
ELISA's sensitivity:
< 10pg/ml
Gene tested:
murine CD32
Latin name:
Mus musculus
Antigen:
NSO, T40-R217
Anti-coagulation:
heparin or EDTA
ELISA kit detection limits:
31.2pg/ml to 2000pg/ml
Target of this ELISA:
Natural and recombinant mouse CD32
Gene name:
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Uniprot:
Low affinity immunoglobulin gamma Fc region receptor II-b
Cross reaction:
the ELISA does not cross react with any other proteins except from closely related species
Detector and capure antibodies:
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
Keep at:
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Properties:
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA's application:
For quantitative measurement of mouse CD32 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
ELISA standard curve:
Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.019 + 0.026 + 0.056 + 0.099 + 0.231 + 0.542 + 1.350 + 2.536
ELISA's precision parameters:
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);195;472;1023;216;515;1174 ELISA standard deviations 8.19;22.66;521.7;11.88;31.93;81 CV(%);4.2;4.8;5.1;5.5;6.2;6.9
More gene info:
CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibodies production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
How the elisa works:
Sample the amount of 100ul/well of the standard solution at 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse CD32 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse CD32 standard solution and each sample be measured in duplicate.
The ELISA contains:
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse CD32 antibodies;1 ST0000-10;Lyophilized recombinant mouse CD32 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse CD32 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Test:
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
ELISA kit description:
GENTAUR's mouse CD32 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for CD32 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, T40-R217) and your samples are measured with a biotin conjugated polyclonal from goat specific for CD32 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse CD32 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Alternative:
CD 32;CD32;CD32 antigen;CD32A;CD32B;CD32C;CDw32;Fc fragment of IgG low affinity II receptor for (CD32);Fc fragment of IgG low affinity IIa receptor;Fc fragment of IgG low affinity IIa receptor for (CD32);Fc fragment of IgG low affinity IIb receptor;Fc fragment of IgG low affinity IIb receptor for (CD32);Fc fragment of IgG low affinity IIc receptor;Fc fragment of IgG low affinity IIc receptor for (CD32);Fc gamma receptor IIB;Fc gamma RII a;Fc gamma RII b;Fc gamma RII c;Fc gamma RIIa;Fc gamma RIIb ;Fc gamma RIIc;Fc receptor, IgG, low affinity IIb;Fc-gamma RII-a;Fc-gamma RII-b;Fc-gamma RII-c;Fc-gamma-RIIa;FC-gamma-RIIB;Fc-GAMMA-RIIC;Fc[g]RII;FCG 2;FCG2;FCG2A_HUMAN;FcgammaRIIC;FCGR 2;FcGR;FCGR2;FCGR2A;FCGR2A1;FCGR2B;FCGR2C;Fcgr3;Fcgr3a;FcgRII;Fcr-2;Fcr-3;FcRII a;FCRII;FcRII b;FcRII c;FcRII-a;FcRIIC;IGFR 2;IGFR2;IgG Fc receptor II a;IgG Fc receptor II b;IgG Fc receptor II beta;IgG Fc receptor II c;IgG Fc receptor II-a;IgG Fc receptor II-b;IgG Fc receptor II-c;Immunoglobulin G Fc receptor II;Low affinity;immunoglobulin gamma Fc region receptor II a;Low affinity immunoglobulin gamma Fc region receptor II;Low affinity immunoglobulin gamma Fc region receptor II b;Low affinity immunoglobulin gamma Fc region receptor II c;Low affinity immunoglobulin gamma Fc region receptor II-a;Low affinity immunoglobulin gamma Fc region receptor II-b;Low affinity immunoglobulin gamma Fc region receptor II-c;Ly-17;Ly-m20;LyM-1;Lymphocyte antigen 17;MGC23887;MGC30032