hydrogen exchanger 3 Antibody, FITC Conjugated

Modification site:

None

Conjugation:

FITC

Tested applications:

IF(IHC-P)

French translation:

anticorps

Modification:

Unmodified

Clonality:

Polyclonal

Excitation Emission:

494nm/518nm

Concentration:

1ug per 1ul

Subcellular location:

Extracellular

Crossreactivity:

Human, Mouse, Rat

Additional conjugation:

Anti-FITC Antibody

Clone:

Polyclonal antibody

Recommended dilutions:

IF(IHC-P)(1:50-200)

Target Antigen:

hydrogen exchanger 3

Category:

Conjugated Primary Antibodies

Host Organism:

Rabbit (Oryctolagus cuniculus)

Purification method:

This antibody was purified via Protein A.

Source:

KLH conjugated synthetic peptide derived from human NHE3

Long name:

hydrogen exchanger 3 Polyclonal Antibody, FITC Conjugated

Specificity:

This is a highly specific antibody against hydrogen exchanger 3.

Crossreactive species details:

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions:

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms:

Na+/H+ exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Sodium; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 sodium/hydrogen exchanger, isoform 3; Solute carrier family 9 sodium/hydrogen exchanger, member 3; Solute carrier family 9 member 3; MGC126718; MGC126720.

Antigen background:

NHE-3 are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells.

Properties:

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.