hydrogen exchanger 3 Antibody, Biotin Conjugated

Excitation Emission:

N/A

Modification site:

None

Conjugation:

Biotin

Tested applications:

WB, IHC-P

French translation:

anticorps

Modification:

Unmodified

Clonality:

Polyclonal

Concentration:

1ug per 1ul

Additional conjugation:

Biotinylated

Subcellular location:

Extracellular

Crossreactivity:

Human, Mouse, Rat

Clone:

Polyclonal antibody

Target Antigen:

hydrogen exchanger 3

Category:

Conjugated Primary Antibodies

Host Organism:

Rabbit (Oryctolagus cuniculus)

Recommended dilutions:

WB(1:100-1000), IHC-P(1:100-500)

Purification method:

This antibody was purified via Protein A.

Source:

KLH conjugated synthetic peptide derived from human NHE3

Long name:

hydrogen exchanger 3 Polyclonal Antibody, Biotin Conjugated

Specificity:

This is a highly specific antibody against hydrogen exchanger 3.

Crossreactive species details:

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions:

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms:

Na+/H+ exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Sodium; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 sodium/hydrogen exchanger, isoform 3; Solute carrier family 9 sodium/hydrogen exchanger, member 3; Solute carrier family 9 member 3; MGC126718; MGC126720.

Properties:

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Antigen background:

NHE-3 are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells.