hydrogen exchanger 3 Antibody, Cy3 Conjugated

Conjugation:

Cy3

Modification site:

None

Tested applications:

IF(IHC-P)

French translation:

anticorps

Modification:

Unmodified

Clonality:

Polyclonal

Concentration:

1ug per 1ul

Subcellular location:

Extracellular

Crossreactivity:

Human, Mouse, Rat

Excitation Emission:

512,550nm/570,615nm

Clone:

Polyclonal antibody

Recommended dilutions:

IF(IHC-P)(1:50-200)

Additional conjugation:

cy3 conjugation kit

Target Antigen:

hydrogen exchanger 3

Category:

Conjugated Primary Antibodies

Host Organism:

Rabbit (Oryctolagus cuniculus)

Purification method:

This antibody was purified via Protein A.

Source:

KLH conjugated synthetic peptide derived from human NHE3

Long name:

hydrogen exchanger 3 Polyclonal Antibody, Cy3 Conjugated

Specificity:

This is a highly specific antibody against hydrogen exchanger 3.

Crossreactive species details:

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions:

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms:

Na+/H+ exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Sodium; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 sodium/hydrogen exchanger, isoform 3; Solute carrier family 9 sodium/hydrogen exchanger, member 3; Solute carrier family 9 member 3; MGC126718; MGC126720.

Properties:

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-hydrogen exchanger 3 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.

Antigen background:

NHE-3 are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells.