Affinity Purified Bovine Type II Collagen Substrate antibody conjugated to FITC

Clone:

N/A

Antibody is raised in:

N/A

Antibody's reacts with:

N/A

Antibody's reacts with these species:

N/A

Antibody's specificity:

N/A

Storage:

-20ºC

Research interest:

Biology

Protein number:

see ncbi

French translation:

anticorps

Application:

Substrate

Category:

Purified Protein

Conjugation:

Anti-FITC Antibody

Antibody come from:

Bovine articular cartilage

Antibody's suited for:

Used for the assay of mammalian collagenase activity.

Antigen-antibody binding interaction:

Affinity Purified Bovine Type II Collagen Substrate antibody conjugated to FITC

Description:

1 of protein A or G purified by nordc will give more specificity than crude serum.

Other description:

Provided as a 1 mg/ml solution in 0.01M acetic acid. DEAE Anion Exchange Chromatography

Gene:

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

Test:

A high affinity purification column was use to purify II Collagen Substrate antibody conjugated FITC by nordc by chromatographic size exclusion.

Warnings:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Long description:

FITC-labeled bovine type II collagen substrate is an excellent substrate for examining collagenase activity. This labelled substrate is highly purified and telo-peptide free and is supplied as solution in acetic acid. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography._x000B__x000B_FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measured the flourescence at 520 nm (Emission)/490 nm (Excitation).

Relevant references:

1. Terato, K., et al. 'A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrate.' Biochim. Biophys. Acta 1976, 445, 753-762_x000B__x000B_2. Sellers, A., et al. 'Evidence that latent collagenases are enzyme-inhibitor complexes.' Biochem. J. 1977, 163, 303-307_x000B__x000B_3. Shinkai, H., et al. 'A complex of collagenase with low molecular weight inhibitors in the culture medium of embryonic chick skin explants.' J. Biochem. (Tokyo) 1977, 81, 261-263_x000B__x000B_4. Shinkai, H. & Nagai, Y., 'A latent collagenase from embryonic human skin explants.' J. Biochem. (Tokyo) 1977, 81, 1261-1263_x000B__x000B_5. Murawaki, Y., et al. 'Serum collagenase activity in patients with chronic liver disease.' J. Hepatol. 1993, 328-334

Properties:

If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.