Rabbit anti Sodium/hydrogen exchanger 1

Antibody's reacts with:

Human

Antibody is raised in:

Rabbit

Storage:

-80ºC

Protein number:

see ncbi

Clone:

Polyclonal

Application:

Western Blot

Antibody's specificity:

No Data Available

Category:

Primary Antibodies

Latin name:

Oryctolagus cuniculus

Antigen-antibody binding interaction:

Rabbit anti Sodium/hydrogen exchanger 1 Antibody

Antibody come from:

Synthetic peptide derived from human NSMAF protein.

Antibody's reacts with these species:

This antibody doesn't cross react with other species

Other description:

Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation

Description:

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Antibody's suited for:

Antibody can be used for Western blotting (1:400 starting dilution) and immunohistochemistry (10-15 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Warnings:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Long description:

NHE-1 is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. NHE-1 plays an important role in signal transduction. Located in the kidney and intestine.

About:

Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Relevant references:

1. OÕBrien, N.W., et al. 'Factor associated with neutral sphingomyelinase activation and its role in cardiac cell death.' Circ Res. 2003 Apr 4;92(6):589-91._x000B__x000B_2. Segui, B., et al. 'CD40 Signals apoptosis through FAN-regulated activation of the sphinogmyelin-ceramide pathway.' J Biol Chem. 1999; 274: 37251Ð37258_x000B__x000B_3. Adam-Klages, S., et al. 'FAN, a novel WD-repeat protein, couples the p55 TNF-receptor to neutral sphingomyelinase.' Cell. 1996; 86: 937Ð947._x000B__x000B_4. Segui, B., et al. 'Involvement of FAN in TNF-induced apoptosis.' J Clin Invest. 2001; 108: 143Ð151.