hydrogen exchanger 3 Antibody, ALEXA FLUOR 488

Modification Site:

None

French translation:

anticorps

Tested applications:

IF(IHC-P)

Clonality:

Polyclonal

Modification:

Unmodified

Concentration:

1ug per 1ul

Conjugation:

Alexa Fluor

Excitation emission:

499nm/519nm

Subcellular location:

Extracellular

Conjugated with:

ALEXA FLUOR® 488

Crossreactivity:

Human, Mouse, Rat

Recommended dilutions:

IF(IHC-P)(1:50-200)

Clone:

Polyclonal antibody

Target Antigen:

hydrogen exchanger 3

Purification:

Purified by Protein A.

Category:

Conjugated Primary Antibodies

Host Organism:

Rabbit (Oryctolagus cuniculus)

Also known as:

Anti-hydrogen exchanger 3 PAb ALEXA FLUOR 488

Specificity:

This is a highly specific antibody against hydrogen exchanger 3.

Long name:

hydrogen exchanger 3 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated

Cross-reactive species details:

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source:

This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human NHE3

Storage conditions:

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Synonyms:

Na+/H+ exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Sodium; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 sodium/hydrogen exchanger, isoform 3; Solute carrier family 9 sodium/hydrogen exchanger, member 3; Solute carrier family 9 member 3; MGC126718; MGC126720.

Properties:

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-hydrogen exchanger 3 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen:

NHE-3 are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells.