Rabbit Anti-Human MARK3 (pThr507) Polyclonal: FITC

Net weight (g):

0.1

Total weight (kg):

1.4

Gene number:

4140

Antibody's reactivity:

Human

Verified applications:

WB, AM

Protein number:

P27448

Raised in:

Rabbit

Storage recommendations:

-20°C

Country of production:

Canada

Stock availabilit:

In Stock

Released date:

29/Aug/2016

Antibody in cell:

Cytoplasmic

Tissue specificity:

Ubiquitous.

Tested reactivity:

To be tested

Tested applications:

To be tested

NCBI number:

NP_001122390

Warning information:

Non-hazardous

PubMed number:

Refer to PubMed

Bibliography:

Refer to PubMed

Shipping recommendations:

Blue Ice or 4°C

Representative figure link:

No Data Available

Conjugation:

Anti-FITC Antibody

Antibody's specificity:

Detects 87.005 kDa.

Antibody's category:

Polyclonal Antibodies

Latin name:

Oryctolagus cuniculus

Antibody's target:

Human MARK3 (pThr507)

Antibody's purified from:

Peptide Affinity Purified

Group:

Polyclonals and antibodies

Recommended buffer for storage:

PBS pH7.4, 50% glycerol, 0.025% Thimerosal

Antibody's full description:

Rabbit Anti-Human MARK3 (pThr507) Polyclonal

Antibody's concentration:

See included datasheet or contact our support service

Scientific context:

See included datasheet or contact our support service.

Antibody's recommended dilutions for use:

WB (1:250); optimal dilutions for assays should be determined by the user.

Antibody's datasheet:

Contact our support service to receive datasheet or other technical documentation.

Test:

StressMark antibodies supplies antibodies that are for research of human proteins.

Immunogen:

A phospho-specific peptide corresponding to residues surrounding Thr507 of human MARK3 (AA504-510)

Description:

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Certificate of analysis:

A 1:250 dilution of SPC-1019 was sufficient for detection of MARK3 (pThr507) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody's other name:

C-TAK1 antibody, Cdc25C associated protein kinase 1 antibody, cTAK1 antibody, ELKL motif kinase 2 antibody, EMK-2 antibody, ETK 1 antibody, MAP microtubule affinity regulating kinase 3 antibody, MAP/microtubule affinity-regulating kinase 3 antibody, Mark3 antibody, MARK3_HUMAN antibody, Par 1a antibody, PAR1A antibody, Protein kinase STK10 antibody, Ser/Thr protein kinase PAR-1 antibody, Serine threonine protein kinase p78 antibody

About:

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Properties:

This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.