GLUC, beta-glucosidase, Affinity purified

Possible reactivity:

n.a.

Raised in:

Rabbit

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

see datasheet

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

Verified reactivity:

Prunus dulcis

Purification:

Affinity purified IgG

References:

to be added when available

Available ordering format:

Lyophilized in phosphate buffer saline

Immunogen:

native beta- glucosidase purified from almonds

Recommended dilutions for use:

1: 1000 - 1: 100 000 for techniques listed above

How to reconstitute:

For reconstitution please add 0.5 ml of sterile destilled water.

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Verified applications:

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Supplementary information:

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Test:

A high affinity purification column was use to purify GLUC, beta-glucosidase, by agisera by chromatographic size exclusion.

Connected products:

AS09 450 | anti-beta glucosidase (biotinylated)AS09 449 | anti-beta glucosidase (total IgG fraction)Plant and algal protein extraction bufferSecondary antibodies

Notes:

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Scientific context:

beta-glucosidase (EC=3.2.1.21) is an enzyme which catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose acting upon upon β1->4 bonds linking two glucose or glucose-substituted molecules.

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.