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Molecular weight (expected | аpparent):59.5 | 45 kDa
Verified applications:ELISA (ELISA), western blot (WB)
Verified reactivity:Arabidopsis thaliana, Kandelia obovata
Recommended dilutions for use:1: 1000 with standard ECL (WB), 1: 8000 (ELISA)
Supplementary information:Contact Gentaur support center at firstname.lastname@example.org
How to reconstitute:See included datasheet or contact our support service
No reactivity:mangrove plants, sp. Avicennia, Nicotiana benthamiana
Notes:Protocol for vacuolar membrane isolation can be found here.
Connected products:collection of antibodies to tonoplast proteins Plant protein extraction bufferSecondary antibodies
Possible reactivity:dictos including: Gossypium hirsutum, Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Populus euphratica
Storage condition:store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Scientific context:Na(+)/H(+) exchanger 1 protein is involved in exchange of protons for cations such as sodium and potassium across membraes. Localized in tonoplast, possibily also to ER and Golgi. Alternative name: NHE-1, Na(+)/H(+) exchanger 1
References:Chen et al. (2013). Nitric Oxide Mediates Root K+/Na+ Balance in a Mangrove Plant, Kandelia obovata, by Enhancing the Expression of AKT1-Type K+ Channel and Na+/H+ Antiporter under High Salinity. 8(8): e71543. doi:10.1371/journal.pone.0071543.
Immunogen:KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NHX protein UniProt: Q68KI4, TAIR: At5g27150; chosen peptide is perfectly confirmed in AtNHX1 UniProt: Q0WVZ5, partially in AtNHX2, UniProt: Q56XP4, and not conserved in AtNHX3, UniProt: Q84WG1 and AtNHX4, UniProt:Q8S397 isoforms