GLYR, Glyoxylate reductase, Affinity purified

Possible reactivity:

n.a.

Raised in:

Rabbit

Molecular weight (expected | аpparent):

30.6 kDa

Clonality:

Polyclonal

Clone:

Polyclonal

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

Verified reactivity:

Spinacia oleracea

Purification:

Affinity purified IgG

References:

to be added when available

Available ordering format:

Lyophilized in phosphate buffer saline

Recommended dilutions for use:

1: 1000 - 1: 8 000 for techniques listed above

Immunogen:

native glyoxylate reductase purified from spinach

How to reconstitute:

For reconstitution please add 0.5 ml of sterile destilled water.

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Verified applications:

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Supplementary information:

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Test:

A high affinity purification column was use to purify GLYR, Glyoxylate reductase, by agisera by chromatographic size exclusion.

Connected products:

AS09 542 | anti-glyoxylate reductaseAS09 543 | anti-glyoxylate reductase(biotinylated)Plant protein extraction bufferSecondary antibodies

Notes:

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Scientific context:

Glyoxylate reductase (EC=1.1.1.26) is an enzyme from oxidoreductase family and participates in glyoxylate and dicarboxylate metabolism. Alternative names: 3-hydroxybutyrate dehydrogenase/ phosphogluconate dehydrogenase (decarboxylating).

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.