Bromelain, Bromelain, Affinity purified

Raised in:

Rabbit

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

see datasheet

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

Verified reactivity:

Ananas comosus

Possible reactivity:

Ananas comosus

Purification:

Affinity purified IgG

References:

to be added when available

Available ordering format:

Lyophilized in phosphate buffer saline

Recommended dilutions for use:

1: 1000 - 1: 100 000 for techniques listed above

Immunogen:

native bromelain isolated and purified from Ananas comosus

How to reconstitute:

For reconstitution please add 0.5 ml of sterile destilled water.

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Scientific context:

Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.

Verified applications:

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Supplementary information:

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Test:

A high affinity purification column was use to purify Bromelain, Bromelain, by agisera by chromatographic size exclusion.

Notes:

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Connected products:

AS09 552 | anti-bromelainAS09 553 | anti-bromelain (biotinylated)AS09 546 | anti-PPI | papain (biotinylated)AS09 547 | anti-PPI | papain (affinity purified)Plant protein extraction bufferSecondary antibodies

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.