Molecular weight (expected | аpparent):see datasheet
TAIR number:Refer to NCBI
Protein number:Refer to NCBI
Verified reactivity:Ananas comosus
Possible reactivity:Ananas comosus
Purification:Affinity purified IgG
References:to be added when available
Available ordering format:Lyophilized in phosphate buffer saline
Recommended dilutions for use:1: 1000 - 1: 100 000 for techniques listed above
Immunogen:native bromelain isolated and purified from Ananas comosus
How to reconstitute:For reconstitution please add 0.5 ml of sterile destilled water.
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Scientific context:Bromelain (EC=126.96.36.199) is a cysteine protease isolated from pineapple stem.
Verified applications:western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Description:1 of protein A or G purified by agisera will give more specificity than crude serum.
Supplementary information:Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
Test:A high affinity purification column was use to purify Bromelain, Bromelain, by agisera by chromatographic size exclusion.
Notes:antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Connected products:AS09 552 | anti-bromelainAS09 553 | anti-bromelain (biotinylated)AS09 546 | anti-PPI | papain (biotinylated)AS09 547 | anti-PPI | papain (affinity purified)Plant protein extraction bufferSecondary antibodies
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.