Molecular weight (expected | аpparent):91 kDa
TAIR number:Refer to NCBI
Protein number:Refer to NCBI
Purification:Affinity purified IgG
References:to be added when available
Available ordering format:Lyophilized in phosphate buffer saline
Verified reactivity:Canavalia ensiformis (common jack-bean)
Recommended dilutions for use:1: 100 - 1: 20 000 for techniques listed above
How to reconstitute:For reconstitution please add 0.5 ml of sterile destilled water.
Possible reactivity:dicots including: Arabidopsis thaliana, Glycine max, Ricinus communis
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Immunogen:urease isolated and purified from Canavalia ensiformis (common jack-beans)
Verified applications:western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Description:1 of protein A or G purified by agisera will give more specificity than crude serum.
Supplementary information:Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
Connected products:AS09 559 | anti-ureaseAS09 560 | anti-urease (biotinylated)Plant protein extraction bufferSecondary antibodies
Test:A high affinity purification column was use to purify Urease, Urease, by agisera by chromatographic size exclusion.
Scientific context:Urease (EC=22.214.171.124) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Notes:antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.