Molecular weight (expected | аpparent):26.9 kDa
TAIR number:Refer to NCBI
Protein number:Refer to NCBI
Purification:Affinity purified IgG
Verified reactivity:Rhodopacter shpaeroides
References:to be added when available
Possible reactivity:Sagittula stellata, Silicibacter sp.
Available ordering format:Lyophilized in phosphate buffer saline
Recommended dilutions for use:1: 100 - 1: 1000 for techniques listed above
How to reconstitute:For reconstitution please add 0.5 ml of sterile destilled water.
No reactivity:no confirmed exceptions from predicted reactivity known in the moment
Immunogen:3-hydroxybutyrate dexydrogenase isolated and purified from Rhodobacter sphaeroides
Verified applications:western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Description:1 of protein A or G purified by agisera will give more specificity than crude serum.
Supplementary information:Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
Test:A high affinity purification column was use to purify 3-hyrdoxybutyrate, dehydrogenase, by agisera by chromatographic size exclusion.
Connected products:AS09 562 | anti-3-hydroxybutyrate dexydrogenase AS09 563 | anti-3-hydroxybutyrate dexydrogenase (biotinylated)Plant protein extraction bufferSecondary antibodies
Notes:antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Scientific context:3-hydroxybutyrate dehydrogenase (EC=126.96.36.199) is an oxidoreductase which participates in synthesis and degradation of ketone bodies and butanoate metabolism. Alternative names: NAD+-beta-hydroxybutyrate dehydrogenase, hydroxybutyrate oxidoreductase, beta-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, -D-hydroxybutyrate dehydrogenase, and beta-hydroxybutyric dehydrogenase.