3-hyrdoxybutyrate, dehydrogenase, Affinity purified

Raised in:

Rabbit

Molecular weight (expected | аpparent):

26.9 kDa

Clonality:

Polyclonal

Clone:

Polyclonal

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

Purification:

Affinity purified IgG

Verified reactivity:

Rhodopacter shpaeroides

References:

to be added when available

Possible reactivity:

Sagittula stellata, Silicibacter sp.

Available ordering format:

Lyophilized in phosphate buffer saline

Recommended dilutions for use:

1: 100 - 1: 1000 for techniques listed above

How to reconstitute:

For reconstitution please add 0.5 ml of sterile destilled water.

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Immunogen:

3-hydroxybutyrate dexydrogenase isolated and purified from Rhodobacter sphaeroides

Verified applications:

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Supplementary information:

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.

Test:

A high affinity purification column was use to purify 3-hyrdoxybutyrate, dehydrogenase, by agisera by chromatographic size exclusion.

Connected products:

AS09 562 | anti-3-hydroxybutyrate dexydrogenase AS09 563 | anti-3-hydroxybutyrate dexydrogenase (biotinylated)Plant protein extraction bufferSecondary antibodies

Notes:

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Storage condition:

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Scientific context:

3-hydroxybutyrate dehydrogenase (EC=1.1.1.30) is an oxidoreductase which participates in synthesis and degradation of ketone bodies and butanoate metabolism. Alternative names: NAD+-beta-hydroxybutyrate dehydrogenase, hydroxybutyrate oxidoreductase, beta-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, -D-hydroxybutyrate dehydrogenase, and beta-hydroxybutyric dehydrogenase.