Size

2 mg

Catalog no.

AS10 1423

Price

262 EUR

Group:

Sera

Raised in:

Goat

Available ordering format:

Liquid

Storage condition:

2-8°C.

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

see datasheet

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

References:

Refer to PubMed

Verified reactivity:

to be determined

Possible reactivity:

to be determined

No reactivity:

to be determined

Conjugation:

not conjugated

Purification:

Affinity purified IgG

Verified applications:

Contact Gentaur support center

Immunogen:

Purified mouse IgG (H&L) AAA51107

Latin name:

Capra aegagrus hircus,Oryctolagus cuniculus

Supplementary information:

Contact Gentaur support center at info@gentaur.com

How to reconstitute:

See included datasheet or contact our support service

Connected products:

other secondary antibodies against anti-mouse IgG (H&L)

Recommended dilutions for use:

This antibody is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.

Test:

A high affinity purification column was use to purify Goat, Unconjugated, min. cross-reactivity , rabbit serum by agisera by chromatographic size exclusion.

Scientific context:

Goat anti-mouse IgG (H&L) is a secondary antibody which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L).

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

About:

Pigs and the smaller guinea pigs are frequent used as models for humans.Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Serum should be stored at +5°C

Notes:

Purity of this preparation is> 95% based on SDS-PAGE. Antibody concentration is> 4.5 mg/ml (E 1% at 280 nm = 14.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins,serum proteins from bovine, horse, human, pig, or rabbit.

Properties:

Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.