Goat, Affinity purified, Unconjugated, min. cross-reactivity to human, mouse, rat serum

Group:

Sera

Raised in:

Goat

Available ordering format:

Liquid

Storage condition:

2-8°C.

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

see datasheet

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

References:

Refer to PubMed

Verified reactivity:

to be determined

Possible reactivity:

to be determined

No reactivity:

to be determined

Conjugation:

not conjugated 

Purification:

Affinity purified IgG

Immunogen:

Purified rabbit IgG (H&L)

Verified applications:

Contact Gentaur support center

Supplementary information:

Contact Gentaur support center at [email protected]

Latin name:

Capra aegagrus hircus,Mus musculus,Rattus norvegicus

How to reconstitute:

See included datasheet or contact our support service

Connected products:

other unconjugated secondary antibodies against rabbit IgG (H&L)

Recommended dilutions for use:

This antibody is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.

Scientific context:

Goat anti-rabbit IgG (H&L) is a secondary antibody which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).

About:

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.Serum should be stored at +5°C

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.

Notes:

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 14.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.

Test:

A high affinity purification column was use to purify Goat, Unconjugated, min. cross-reactivity mouse, serum by agisera by chromatographic size exclusion.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.