Available ordering format:Liquid
Molecular weight (expected | аpparent):see datasheet
TAIR number:Refer to NCBI
Protein number:Refer to NCBI
References:Refer to PubMed
Verified reactivity:to be determined
Possible reactivity:to be determined
No reactivity:to be determined
Latin name:Oryctolagus cuniculus
Immunogen:Purified rat IgG (H&L)
Purification:Affinity purified Rabbit IgG
Verified applications:Contact Gentaur support center
Supplementary information:Contact Gentaur support center at firstname.lastname@example.org
How to reconstitute:See included datasheet or contact our support service
Connected products:other unconjugated secondary antibodies against rat IgG (H&L)
Recommended dilutions for use:This antibody is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
Test:A high affinity purification column was use to purify Rabbit, Unconjugated, min. cross-reactivity serum by agisera by chromatographic size exclusion.
Scientific context:Rabbit anti-rat IgG (H&L) is a secondary antibody which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Properties:Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
About:Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Serum should be stored at +5°C
Notes:Purity of this preparation is> 95% based on SDS-PAGE. Antibody concentration is> 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins
Description:1 of protein A or G purified by agisera will give more specificity than crude serum.Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.