Size

1 mg

Catalog no.

AS10 666

Price

312 EUR

Raised in:

Goat

Available ordering format:

Liquid

Clonality:

Polyclonal

Clone:

Polyclonal

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

Molecular weight (expected | аpparent):

see datasheet

Conjugation:

not conjugated

Latin name:

Capra aegagrus hircus

References:

to be added when available

Purification:

Affinity purified goat IgG

Immunogen:

purified biotin, whole molecule

Supplementary information:

No reactivity is observed to KLH or BSA

Verified reactivity:

free biotin, biotin coupled to KLH and BSA

Possible reactivity:

free biotin, biotin coupled to KLH and BSA

How to reconstitute:

See included datasheet or contact our support service

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Verified applications:

ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)

Connected products:

AS09 607 | Goat anti-rabbit IgG (H&L) AP conjugatedAS09 608 | Goat anti-rabbit IgG (H&L) biotin conjugated

Scientific context:

Biotin is a water soluble B-complex vitamin. Chemically linked to proteins videly used in biochemical assays.

Storage condition:

Store at 2-8°C. Prepare working dilution prior to use and then discard. Stable for 1 year from date or receipt.

Recommended dilutions for use:

1: 100 -1: 10 000 for coating (ELISA), 1: 1000 -1: 30 000 as primary (ELISA), 1: 100 to 1: 5000 for other techniques

Test:

A high affinity purification column was use to purify Goat, Unconjugated by agisera by chromatographic size exclusion.

Notes:

Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.20.1 % (w/v) sodium azide is used as preservative.