Goat, IgM, affinity purified

Additional isotype:

IgM

Raised in:

Goat

Available ordering format:

Liquid

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

see datasheet

TAIR number:

Refer to NCBI

Protein number:

Refer to NCBI

Verified applications:

ELISA (ELISA)

Immunogen:

Purified human IgG

Latin name:

Capra aegagrus hircus

Purification:

Affinity purified goat IgG

References:

to be added when available

Recommended dilutions for use:

needs to be determined by end user

Connected products:

other secondary antibodies to human immunoglobulins

Verified reactivity:

human IgG Fc (two heavy chains with constant domains)

How to reconstitute:

See included datasheet or contact our support service

Possible reactivity:

human IgG Fc (two heavy chains with constant domains),human IgG (H&L)

No reactivity:

no confirmed exceptions from predicted reactivity known in the moment

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Test:

A high affinity purification column was use to purify Goat, IgM, by agisera by chromatographic size exclusion.

Notes:

Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.

Scientific context:

Goat anti-human IgG Fc (two heavy chains with constant domains)is a secondary antibody which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.

Supplementary information:

Antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to light chains on human immunoglobulins and to human IgA and IgM based on immunoelectrophoresis.

Properties:

Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

Storage condition:

Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.