Molecular weight (expected | аpparent):16 kDa
Available ordering format:Lyophilized
Recommended dilutions for use:1 : 1000 (WB)
Verified applications:western blot (WB)
Verified reactivity:Arabidopsis thaliana
Purification:Affinity purified serum in PBS pH 7.4
How to reconstitute:For reconstitution add 50 µl of sterile water.
Supplementary information:Contact Gentaur support center at firstname.lastname@example.org
References:to be added when available, antibody released in August 2016.
No reactivity:no confirmed exceptions from predicted reactivity are currently known
Description:1 of protein A or G purified by agisera will give more specificity than crude serum.
Possible reactivity:Arachis hypogaea, Brassica sp., Glycine max, Medicago truncatula, Phaseolus vulgaris, Thellungiella halophila
Immunogen:recombinant LEA4-5 from Arabidopsis thaliana: UniProt: Q9FG31, TAIR: AT5G06760, a group 4 LEA protein (Battaglia et al., 2008)
Test:A high affinity purification column was use to purify LEA4-5, Late embryogenesis abundant protein 4-5 ( ) by agisera by chromatographic size exclusion.
Connected products:AS13 2758 | anti-LEA4-5 | Late embryogenesis abundant protein 4-5, rabbit antibodyAS13 2756 | Anti-LEA6-3 | late embryogenesis abundant protein 6-3, rabbit antibodyPlant protein extraction buffer Secondary antibodies
Storage condition:store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Scientific context:LEA (Late embryogenesis abundant) proteins are very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.