GFP, Green Fluorescence Protein (affinity purified)

Notes:

None

Raised in:

Rabbit

Protein number:

P42212

Available ordering format:

Inquire

Clonality:

Polyclonal

Clone:

Polyclonal

Molecular weight (expected | аpparent):

see datasheet

TAIR number:

Refer to NCBI

No reactivity:

to be determined

Possible reactivity:

to be determined

Purification:

Affinity purified serum

Supplementary information:

Minimal cross-reactivity with E.coli proteins.

How to reconstitute:

For reconstitution add 100 µl of sterile water

Verified reactivity:

native GFP, recombinant GFP (E.coli), all variants of GFP

Verified applications:

ELISA (ELISA), Western blot (WB), Immunofluorescence (IF)

References:

to be added when available, antibody released in October 2015

Recommended dilutions for use:

1: 5000 - 1: 25 000 (ELISA), 1: 500 (IF), 1: 2000 - 1: 10 000 (WB)

Immunogen:

highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Description:

1 of protein A or G purified by agisera will give more specificity than crude serum.

Connected products:

AS15 3000 | GFP (protein A purified), rabbit antibodyAS15 2999 | GFP (total IgG), rabbit antibodyAS15 3001 | GFP, peroxidase conjugated, rabbit antibodyAS15 2998 | GFP (total IgY), chicken antibodyAS15 3002 | GFP (purified recombinant protein)other tag antibodies

Storage condition:

store lyophilized/reconstituted at 4°C, or in small aliquots at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Test:

A high affinity purification column was use to purify GFP, Protein ( ) by agisera by chromatographic size exclusion.Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.

Scientific context:

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.