Available ordering format:Inquire
Molecular weight (expected | аpparent):15 | 17 kDa
TAIR number:Refer to NCBI
Purification:Affinity purified serum
Supplementary information:To be added when available
Immunogen:KLH-conjugated synthetic peptide
How to reconstitute:See included datasheet or contact our support service
Possible reactivity:chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.
References:To be added when available, antibody released in March 2016.
No reactivity:No confirmed exceptions from predicted reactivity known at the moment
Recommended dilutions for use:2 µg/million cells (ChIP), 1: 20 000 (Dot), 1: 2000 (IF), 1: 100 (WB)
Description:1 of protein A or G purified by agisera will give more specificity than crude serum.
Verified applications:chromatin immunoprecipiation (ChIP), Dot Blot (Dot), ELISA (ELISA), immunofluorescence (IF), western blot (WB)
Notes:This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0.05% sodium azide and 0.05% ProClin300.
Test:A high affinity purification column was use to purify H3S10p, Histone H3 (p Ser10) ( ) by agisera by chromatographic size exclusion.
Storage condition:Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Scientific context:Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Phosphorylation of H3S10 is associated with mitosis.
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