B-TeZ IAC Biotin Vitamin affinity column

Crossreactivity:

N/A

Not included in the standard package:

N/A

Included in the standard package:

N/A

Antigen:

Biotin

Reacts with:

Biotin

Specificity:

Biotin

Long term storage:

2-8°C

ELISA sensitivity:

0.01 μg

Storage recommendations:

12 month

ELISA detection limits:

0.01 - 5μg

Conjugation:

Biotinylated

Representative figures:

No Available

Shipping and storage conditions:

> 0 to +37°C

Package form:

Column size 3mL

Research area interests:

Vitamin Analysis

PubMed citations:

See the datasheet

Warnings:

For Research Use only.

ELISA category:

Vitamin affinity column

Samples to be analyzed:

food and feed crop probes

Technical datasheet:

Contact us to receive the datasheet

Preservative materials:

If applicable mentioned in the product data sheet

Assay principle:

Enrichment of analytes via immuno affinity chromatography

Test:

A high affinity purification column was use to purify B-TeZ IAC Vitamin column by biotez by chromatographic size exclusion.

ELISA's description:

10 Immuno affinity columns with specific antibodies binding Biotin (Vitamin B7, Vitamin H). For enrichment or purification.

Tested applications:

B-TeZ IAC Biotin is an immuno affinity column (IAC) for enrichment and isolation of Biotin. The IAC can be used after extraction of the analytes.

Properties:

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.