Human CellExp™ FCGR3A/CD16a, human recombinant

Concentration:

N/A

Aa sequence:

N/A

Recombinant:

Yes

Unit:

N/A

Binding ability:

N/A

Tested activity:

N/A

NCBI gene number:

2214

Gene source:

Human

NCBI gene:

FCGR3A

NCBI number:

P08637

Storage condition:

-20°C

Purification:

≥98%

Shipping under:

Gel Pack

Physical appearance:

Lyophilized

Group:

recombinants

Source:

HEK293 cells

Target:

FCGR3A/CD16a

Tested applications:

SDS-PAGE, N/A

Additional source:

Recombinants or rec. proteins

Other name:

FCGR3A, CD16A, FCG3, FCGR3, IGFR3

Synonym:

FCGR3A, CD16A, FCG3, FCGR3, IGFR3

Before use:

Centrifuge the vial prior to opening.

Notes:

For Research Use Only! Not to be used in humans

Reesults:

Measured by its ability to bind human IgG with an estimated KD < 45 nM.

Biological activity:

Measured by its ability to bind human IgG with an estimated KD < 45 nM.

Physical properties:

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Molecular weight:

This protein is fused with 6×his tag at the C-terminus, and has a calculated MW of 22.7 kDa. The predicted N-terminus is Gly 17. DTT-reduced Protein migrates as 36-45 kDa in SDS-PAGE due to glycosylation.

How to reconstitute:

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Properties:

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background:

CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and Fcγ IIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.