Rabbit Polyclonal IgG Anti-Beta-Defensin-1 (HBD-1), affinity purified

Additional isotype:

IgG

Antibody's isotype:

IgG

Protein concentration:

N/A

Antibody's raised in:

Rabbit

Antibody's clone:

polyclonal

Product is supplied as:

lyophilisate

Product category:

Primary Antibody

Storage method:

Store at -20'°C'

Latin name:

Oryctolagus cuniculus

Tested applications:

IHC(C, P), ELISA, RIA

UniProt number:

Go to UniProt/SwissProt

Group:

Polyclonals and antibodies

Positive control:

Renal epithelia, bladder epithelia

Availability:

Please contact us to check the availability

Species reactivity:

human, monkey, no cross-reactivity with mouse and rat

Recommended concentration for use:

1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested)

Immunogen:

Synthetic peptide, 17 AA near N-terminus of the mature HBD-1, coupled to KLH for immunisation of rabbits

Test:

A high affinity purification column was use to purify Research by bio logo by chromatographic size exclusion.

Recommended secondary reagent:

We recommend the VECTASTAIN Elite ABC Kit Rabbit IgG (code no. PK-6101) or BioPrime Universal Staining Kits (code no. AE005 and DA005).

Specificity:

ß-defensin-1 (HBD-1) whole molecule and 17AA N-terminal fraction of HBD-1, no cross-reactivity to alpha-defensin and other beta-defensins

Tips:

Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Product presentation:

Peptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.

Description:

1 of protein A or G purified by bio logo will give more specificity than crude serum.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.

Litterature:

1. Bensch K.W., Raida M., Mägert H.J., Schulz-Knappe P., and W.G. Forssmann. HBD-1: a novel beta -defensin from human plasma. FEBS Lett 1995, 368; 331-335. (original publication HBD-1) 2. Valore EV, Park CH, Quayle AJ, Wiles KR, McCray PB, Ganz T. Human beta-defensin-1: an antimicrobial peptide of urogenital tissues. J Clin Invest. 1998 Apr 15;101(8):1633-42. 3. O'Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T, Kagnoff MF. Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol. 1999 Dec 15;163(12):6718-24. 4. Ganz T. Defensins and host defense. Science. 1999 Oct 15;286(5439):420-1. 5. Shi J, Zhang G, Wu H, Ross C, Blecha F, Ganz T. Porcine epithelial beta-defensin 1 is expressed in the dorsal tongue at antimicrobial concentrations. Infect Immun. 1999 Jun;67(6):3121-7.

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.