Rabbit Polyclonal IgG Anti-Beta-Defensin-1 (MBD-1), affinity purified

Additional isotype:

IgG

Antibody's isotype:

IgG

Protein concentration:

N/A

Species reactivity:

Mouse

Antibody's raised in:

Rabbit

Antibody's clone:

polyclonal

Product is supplied as:

lyophilisate

Product category:

Primary Antibody

Storage method:

Store at -20'°C'

Latin name:

Oryctolagus cuniculus

UniProt number:

Go to UniProt/SwissProt

Group:

Polyclonals and antibodies

Positive control:

Kidney or bladder epithelia

Tested applications:

ELISA, RIA, IHC not established

Availability:

Please contact us to check the availability

Immunogen:

Synthetic 16 aa N-terminal peptide of mouse beta-defensin-1

Recommended concentration for use:

1-10 µg/ml Western Blot, 0.5-1 µg/ml ELISA, IHC 2-10 µg/ml (not tested)

Specificity:

Mouse beta-defensin-11 (MBD-1), cross-reactivity with rat expected, no cross-reaction with HBD-1

Test:

A high affinity purification column was use to purify Research by bio logo by chromatographic size exclusion.

Recommended secondary reagent:

We recommend the VECTASTAIN Elite ABC Kit Rabbit IgG (Ref. PK-6101) or BioPrime Universal Staining Kits (Ref. AE005 and DA005).

Tips:

Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Product presentation:

Peptide Sepharose purified IgG in PBS, BSA, lyophilized without preservative. Reconstitute with 0.1 ml sterile water to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.

Litterature:

1. Huttner KM, Kozak CA, Bevins CL. The mouse genome encodes a single homolog of the antimicrobial peptide human beta-defensin 1. FEBS Lett. 1997 Aug 11;413(1):45-49. 2. Ganz T. Defensins and host defense. Science. 1999 Oct 15;286(5439):420-1.

Description:

1 of protein A or G purified by bio logo will give more specificity than crude serum.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.

About:

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.