Concentration:1ug per 1ul
Conjugated:Alexa conjugate 1
Conjugated with:ALEXA FLUOR® 594
Applications with corresponding dilutions:IF(IHC-P)(1:50-200)
Target Protein/Peptide:hydrogen exchanger 3
Purification method:Purified by Protein A.
Group:Polyclonals and antibodies
Type:Conjugated Primary Antibody
Conjugation:Alexa Fluor,ALEXA FLUOR® 594
Host organism:Rabbit (Oryctolagus cuniculus)
Other name:Anti-hydrogen exchanger 3 Polyclonal
Also known as:hydrogen exchanger 3 Polyclonal Antibody
Properties:For facs or microscopy Alexa 1 conjugate.
Modification:No modification has been applied to this antibody
Antigen Source:KLH conjugated synthetic peptide derived from human NHE3
Specificity:This antibody reacts specifically with hydrogen exchanger 3
Cross reactive species:Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Storage:Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Description:This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About:Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details:No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Synonyms:Na+/H+ exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Sodium; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 sodium/hydrogen exchanger, isoform 3; Solute carrier family 9 sodium/hydrogen exchanger, member 3; Solute carrier family 9 member 3; MGC126718; MGC126720.
Advisory:Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Background information:NHE-3 are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells.